The stem hairpin loop structure of p2Sp1 RNA is required for RNA-cleaving activity.
Identifieur interne : 003776 ( Main/Exploration ); précédent : 003775; suivant : 003777The stem hairpin loop structure of p2Sp1 RNA is required for RNA-cleaving activity.
Auteurs : K. Hosono [Japon] ; H. Hosaka ; G. Kawai ; K. Takai ; H. TakakuSource :
- Biochimica et biophysica acta [ 0006-3002 ] ; 1999.
Descripteurs français
- KwdFr :
- MESH :
- métabolisme : ARN.
- ARN, Cinétique, Conformation d'acide nucléique, Dénaturation d'acide nucléique, Hydrolyse, Séquence nucléotidique.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : RNA.
- chemical , metabolism : RNA.
- Base Sequence, Hydrolysis, Kinetics, Nucleic Acid Conformation, Nucleic Acid Denaturation.
Abstract
We studied the hairpin-loop structure of an RNA fragment (GUUUCGUACAAAC) (R13) with the sequence corresponding to the self-cleavage domain in the precursor of an RNA molecule from bacteriophage T4-infected Escherichia coli cells (p2Sp1 RNA). In order to determine the influence of the hairpin-loop structure on these sequence-specific cleavage reactions, we have synthesized oligoribonucleotides containing hairpin-loop, double-helical stem-loop, and single-stranded RNA structures. The cleavage was affected by the hairpin-loop structure. Furthermore, the helix-stem, which retains the thermodynamically extrastable stem hairpin-loop structures, is also important for the cleavage activity. However, the thermodynamically extrastable helix-stem structure reduced the cleavage activity of the adjacent UA and CA sequences at the helix-stem site. For the cleavage reactions of the RNA cleavage products, the R6 (ACAAAC), R7 (GUUUCGU), and R9 (GUUUCGUAC) mers from the parent RNA, R13 (GUUUCGUACAAAC), a very slight amount of cleavage product (2%) from the RNA 9 was observed, but no reaction occurred for the R6 and R7. We also describe the influences of the sequences (UA and CA) on the cleavage activity.
DOI: 10.1016/s0167-4781(99)00215-8
PubMed: 10673038
Affiliations:
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Le document en format XML
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Kawai</name></author><author><name sortKey="Takai, K" sort="Takai, K" uniqKey="Takai K" first="K" last="Takai">K. Takai</name></author><author><name sortKey="Takaku, H" sort="Takaku, H" uniqKey="Takaku H" first="H" last="Takaku">H. Takaku</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1999">1999</date><idno type="RBID">pubmed:10673038</idno><idno type="pmid">10673038</idno><idno type="doi">10.1016/s0167-4781(99)00215-8</idno><idno type="wicri:Area/PubMed/Corpus">002600</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002600</idno><idno type="wicri:Area/PubMed/Curation">002600</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002600</idno><idno type="wicri:Area/PubMed/Checkpoint">002461</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002461</idno><idno type="wicri:Area/Ncbi/Merge">000045</idno><idno type="wicri:Area/Ncbi/Curation">000045</idno><idno type="wicri:Area/Ncbi/Checkpoint">000045</idno><idno type="wicri:doubleKey">0006-3002:1999:Hosono K:the:stem:hairpin</idno><idno type="wicri:Area/Main/Merge">003820</idno><idno type="wicri:Area/Main/Curation">003776</idno><idno type="wicri:Area/Main/Exploration">003776</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The stem hairpin loop structure of p2Sp1 RNA is required for RNA-cleaving activity.</title><author><name sortKey="Hosono, K" sort="Hosono, K" uniqKey="Hosono K" first="K" last="Hosono">K. Hosono</name><affiliation wicri:level="1"><nlm:affiliation>Department of Industrial Chemistry and High Technology Research Center, Chiba Institute of Technology, Narashino, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Industrial Chemistry and High Technology Research Center, Chiba Institute of Technology, Narashino</wicri:regionArea><wicri:noRegion>Narashino</wicri:noRegion></affiliation></author><author><name sortKey="Hosaka, H" sort="Hosaka, H" uniqKey="Hosaka H" first="H" last="Hosaka">H. Hosaka</name></author><author><name sortKey="Kawai, G" sort="Kawai, G" uniqKey="Kawai G" first="G" last="Kawai">G. Kawai</name></author><author><name sortKey="Takai, K" sort="Takai, K" uniqKey="Takai K" first="K" last="Takai">K. Takai</name></author><author><name sortKey="Takaku, H" sort="Takaku, H" uniqKey="Takaku H" first="H" last="Takaku">H. Takaku</name></author></analytic><series><title level="j">Biochimica et biophysica acta</title><idno type="ISSN">0006-3002</idno><imprint><date when="1999" type="published">1999</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence</term><term>Hydrolysis</term><term>Kinetics</term><term>Nucleic Acid Conformation</term><term>Nucleic Acid Denaturation</term><term>RNA (chemistry)</term><term>RNA (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN ()</term><term>ARN (métabolisme)</term><term>Cinétique</term><term>Conformation d'acide nucléique</term><term>Dénaturation d'acide nucléique</term><term>Hydrolyse</term><term>Séquence nucléotidique</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>RNA</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>RNA</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Hydrolysis</term><term>Kinetics</term><term>Nucleic Acid Conformation</term><term>Nucleic Acid Denaturation</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ARN</term><term>Cinétique</term><term>Conformation d'acide nucléique</term><term>Dénaturation d'acide nucléique</term><term>Hydrolyse</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We studied the hairpin-loop structure of an RNA fragment (GUUUCGUACAAAC) (R13) with the sequence corresponding to the self-cleavage domain in the precursor of an RNA molecule from bacteriophage T4-infected Escherichia coli cells (p2Sp1 RNA). In order to determine the influence of the hairpin-loop structure on these sequence-specific cleavage reactions, we have synthesized oligoribonucleotides containing hairpin-loop, double-helical stem-loop, and single-stranded RNA structures. The cleavage was affected by the hairpin-loop structure. Furthermore, the helix-stem, which retains the thermodynamically extrastable stem hairpin-loop structures, is also important for the cleavage activity. However, the thermodynamically extrastable helix-stem structure reduced the cleavage activity of the adjacent UA and CA sequences at the helix-stem site. For the cleavage reactions of the RNA cleavage products, the R6 (ACAAAC), R7 (GUUUCGU), and R9 (GUUUCGUAC) mers from the parent RNA, R13 (GUUUCGUACAAAC), a very slight amount of cleavage product (2%) from the RNA 9 was observed, but no reaction occurred for the R6 and R7. We also describe the influences of the sequences (UA and CA) on the cleavage activity.</div></front></TEI><affiliations><list><country><li>Japon</li></country></list><tree><noCountry><name sortKey="Hosaka, H" sort="Hosaka, H" uniqKey="Hosaka H" first="H" last="Hosaka">H. Hosaka</name><name sortKey="Kawai, G" sort="Kawai, G" uniqKey="Kawai G" first="G" last="Kawai">G. Kawai</name><name sortKey="Takai, K" sort="Takai, K" uniqKey="Takai K" first="K" last="Takai">K. Takai</name><name sortKey="Takaku, H" sort="Takaku, H" uniqKey="Takaku H" first="H" last="Takaku">H. Takaku</name></noCountry><country name="Japon"><noRegion><name sortKey="Hosono, K" sort="Hosono, K" uniqKey="Hosono K" first="K" last="Hosono">K. Hosono</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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